Variations in Plasmid DNA Sequences Present in Crown Gall Tumour Lines
نویسندگان
چکیده
منابع مشابه
Unusual plasmid DNA organization in an octopine crown gall tumor.
A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization ...
متن کاملRevertant seedlings from crown gall tumors retain a portion of the bacterial Ti plasmid DNA sequences.
BT37 is a crown gall teratoma incited on tobacco by Agrobacterium tumefaciens containing pTi-T37, a nopaline-type Ti plasmid. Treatment of this cloned tumor tissue with kinetin at 1 mg/liter results in the formation of relatively normal-appearing shoots. These shoots can be induced to root and set viable seed. In contrast to BT37 tissue, the derived tissues are not phytohormone independent and ...
متن کاملSizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete polyadenylylated RNAs (sizes 0.73-1.75 kilobases) were detected in octopine-producing tumors (lines A6S/2, E9, and 15955/01). In one tumor (line 15955/1) in which octopine could not be detected, one of th...
متن کاملCrown gall teratoma formation is plasmid and plant controlled.
Experiments using different species of the plant Nicotiana and strains of the bacterium Agrobacterium tumefaciens showed that teratoma formation from crown galls was dependent on the combination of bacterial Ti plasmid and host plant used.
متن کاملAgrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.
Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1977
ISSN: 0022-1287
DOI: 10.1099/00221287-102-2-427